# US 2017/0280668 A1 · Hybrid swiss chard variety 69-104 rz

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- Number: US 2017/0280668 A1
- Document ID: US20170280668A1
- Status: Published application
- Kind: A1
- Application number: 15/466,971
- Filed: 2017-03-23
- Published: 2017-10-05
- Earliest claimed: 2016-03-29
- Assignees: Rijk Zwaan Zaadteelt En Zaadhandel B.V.
- Applicants: Rijk Zwaan Zaadteelt En Zaadhandel B.V.
- Inventors: Franciscus Hermanus Carree
- Web page: https://patentgrep.com/patent/US20170280668A1

## CPC

- A01H 6/024: {Beta vulgaris [beet]}
- A01H 5/12: Leaves
- C12Q 1/6895: for plants, fungi or algae
- C12Q 2600/13: Plant traits
- C12Q 2600/156: Polymorphic or mutational markers
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

The present invention relates to a hybrid Swiss chard seed designated 69-104 RZ. The present invention also relates to a Swiss chard plant produced by growing the 69-104 RZ seed, which plant exhibits a combination of traits including green leaves with a reddish purple petiole and midrib, slow growing, resistance to downy mildew (Peronospora farinosa f sp. betae), a dark to very dark green leaf coloring and strongly blistered leaf blades. The invention further relates to methods for producing the Swiss chard cultivar, represented by hybrid Swiss chard variety 69-104 RZ.

## Claims

### Claim 1

A seed of hybrid Swiss chard variety 69-104 RZ, a sample of seed of said hybrid variety having been deposited under NCIMB Accession Number 42561.

### Claim 2 (depends on 1)

A plant grown from the seed of claim 1.

### Claim 3 (depends on 2)

The Swiss chard plant of claim 2, which is a plant grown from seed having been deposited under NCIMB Accession Number 42561.

### Claim 4 (depends on 2)

A Swiss chard plant, or a part thereof, having all the physiological and morphological characteristics of the Swiss chard plant of claim 2.

### Claim 5 (depends on 2)

A part of the plant of claim 2, wherein said part is a microspore, pollen, an ovary, an ovule, an embryo sac, an egg cell, a cutting, a root, a stem, a cell or a protoplast.

### Claim 6 (depends on 5)

A tissue culture of regenerable cells or protoplasts from the plant part of claim 5.

### Claim 7 (depends on 6)

The tissue culture as claimed in claim 6, wherein said cells or protoplasts of the tissue culture are derived from a leaf, pollen, an embryo, a cotyledon, a hypocotyl, a meristematic cell, a root, a root tip, an anther, a flower, a seed or a stem.

### Claim 8 (depends on 6)

A Swiss chard plant regenerated from the tissue culture of claim 6, wherein the regenerated plant expresses all of the physiological and morphological characteristics of hybrid Swiss chard variety 69-104 RZ, a sample of seed of said hybrid having been deposited under NCIMB Accession Number 42561.

### Claim 9

A method of vegetatively propagating a plant of hybrid Swiss chard variety 69-104 RZ comprising the steps of: (a) collecting tissue capable of being propagated from a plant of hybrid Swiss chard variety 69-104 RZ, representative seed of said hybrid Swiss chard variety having been deposited under NCIMB Accession Number 42561; and (b) producing a rooted plant from said tissue.

### Claim 10 (depends on 2)

A method of producing a Swiss chard seed, comprising crossing the plant of claim 2 with itself or a second Swiss chard plant.

### Claim 11 (depends on 10)

A Swiss chard seed produced by the method of claim 10.

### Claim 12 (depends on 11)

A Swiss chard plant grown from the seed as claimed in claim 11.

### Claim 13

A method for producing a seed of a hybrid Swiss chard variety 69-104 RZ-derived Swiss chard plant comprising the steps of: (a) crossing a Swiss chard plant of hybrid variety 69-104 RZ, representative seed of which having been deposited under NCIMB Accession Number 42561, with a second Swiss chard plant or with itself; and (b) allowing seed of a hybrid variety 69-104 RZ-derived Swiss chard plant to form.

### Claim 14 (depends on 13)

The method of claim 13, further comprising the steps of: (c) selfing the plant grown from said hybrid variety 69-104 RZ-derived Swiss chard seed or crossing it to a second Swiss chard plant to yield additional hybrid variety 69-104 RZ-derived Swiss chard seed; (d) growing said additional hybrid variety 69-104 RZ-derived Swiss chard seed of step (c) to yield additional 69-104 RZ-derived Swiss chard plants; and (e) repeating the crossing and growing steps of (c) and (d) for an additional 3-10 generations to generate further 69-104 RZ-derived Swiss chard plants, and (f) allowing seed of a hybrid variety 69-104 RZ-derived Swiss chard plant to form.

### Claim 15 (depends on 13, 14)

A Swiss chard seed produced by the method of claim 13 or 14.

### Claim 16 (depends on 15)

A Swiss chard plant grown from the seed of claim 15.

### Claim 17

A method of producing a plant of Swiss chard hybrid variety 69-104 RZ comprising at least one new trait, the method comprising introducing a mutation or transgene conferring the at least one new trait into a plant of hybrid Swiss chard variety 69-104 RZ, wherein a sample of seed of said variety has been deposited under NCIMB Accession Number 42561.

### Claim 18 (depends on 17)

The Swiss chard plant produced by the method of claim 17.

### Claim 19 (depends on 2)

A method of producing Swiss chard leaves comprising: (a) obtaining a plant according to claim 2, wherein the plant has been cultivated to obtain leaves; and (b) harvesting Swiss chard leaves from the plant to thereby obtain harvested Swiss chard leaves.

### Claim 20 (depends on 19)

The method of claim 19 further comprising packaging the harvested Swiss chard leaves as a fresh vegetable.

### Claim 21 (depends on 19)

The method of claim 19 further comprising processing the harvested Swiss chard leaves as a processed food.

### Claim 22 (depends on 2)

A container comprising one or more Swiss chard plants of claim 2 for harvest of leaves.

### Claim 23

A method of determining the genotype of a plant of hybrid Swiss chard variety 69-104 RZ, representative seed of which has been deposited under NCIMB Accession Number 42561, or a first generation progeny thereof, comprising obtaining a sample of nucleic acids from said plant and comparing said nucleic acids to a sample of nucleic acids obtained from a reference plant, and detecting a plurality of polymorphisms between the two nucleic acid samples, wherein the plurality of polymorphisms are indicative of hybrid Swiss chard variety 69-104 RZ and/or give rise to the expression of any one or more, or all, of the morphological and physiological characteristics of hybrid Swiss chard variety 69-104 RZ.

### Claim 24

A plant of Swiss chard hybrid variety 69-104 RZ comprising a transgene conferring a desired trait, a sample of seed of said variety has been deposited under NCIMB Accession Number 42561.

### Claim 25 (depends on 24)

A seed that produces the plant of claim 24.