# US 2017/0280666 A1 · Lactuca sativa cultivar cvx-35 > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2017/0280666 A1 - Document ID: US20170280666A1 - Status: Published application - Kind: A1 - Application number: 15/085,277 - Filed: 2016-03-30 - Published: 2017-10-05 - Earliest claimed: 2016-03-30 - Assignees: Central Valley Seeds, Inc. - Applicants: Central Valley Seeds, Inc. - Inventors: Tony M. Avila; Adolfo S. Mederos - Web page: https://patentgrep.com/patent/US20170280666A1 ## CPC - A01H 6/1472: {Lactuca sativa [lettuce]} - A01H 5/12: Leaves - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ## Abstract According to the invention, there is provided a novel romaine lettuce cultivar, designated CVX-35. ‘CVX-35’ is described as a vigorous romaine cultivar recommended for the main lettuce growing regions of California and Arizona. It has larger frame size and heavier weight, short core length, savoyed and glossy leaf color, corky root rot resistance, Bushy Stunt virus resistance, no fringe burn on mature leaves and also yellower heart leaf color. The present invention relates to a new and distinctive cos or romaine lettuce (Lactuca sativa L.) variety designated CVX-35. This invention thus relates to the seeds of lettuce cultivar CVX-35, to the plants of lettuce cultivar CVX-35, to plant parts of lettuce cultivar CVX-35, to methods for producing a lettuce cultivar by crossing the lettuce cultivar CVX-35 with another lettuce cultivar, and to methods for producing a lettuce cultivar containing in its genetic material one or more backcross conversion traits or transgenes and to the backcross conversion lettuce plants and plant parts produced by those methods. ## Claims ### Claim at position 1 1: A seed of lettuce cultivar designated CVX-35, wherein a representative sample of seed of said cultivar was deposited under ATCC Accession No. PTA-______. ### Claim at position 2 (depends on 1) 2: A lettuce plant, or a part thereof, produced by growing the seed of claim 1. ### Claim at position 3 (depends on 2) 3: A tissue culture of cells produced from the plant of claim 2, wherein said cells of the tissue culture are produced from a plant part selected from the group consisting of embryo, meristematic cell, leaf, cotyledon, hypocotyl, stem, root, root tip, pistil, anther, flower, seed and pollen. ### Claim at position 4 (depends on 2) 4: A protoplast produced from the plant of claim 2. ### Claim at position 5 (depends on 3) 5: A protoplast produced from the tissue culture of claim 3. ### Claim at position 6 (depends on 3) 6: A lettuce plant regenerated from the tissue culture of claim 3, wherein the plant has all of the morphological and physiological characteristics of cultivar CVX-35, wherein a representative sample of seed was deposited under ATCC Accession No. PTA-______. ### Claim at position 7 (depends on 2) 7: A method for producing a hybrid lettuce seed, wherein the method comprises crossing the plant of claim 2 with a different lettuce plant and harvesting the resultant F1 hybrid lettuce seed. ### Claim at position 8 (depends on 7) 8: An F1 hybrid lettuce seed produced by the method of claim 7. ### Claim at position 9 (depends on 8) 9: An F1 hybrid lettuce plant, or a part thereof, produced by growing said hybrid seed of claim 8. ### Claim at position 10 (depends on 2) 10: A method of producing a male sterile lettuce plant wherein the method comprises transforming the lettuce plant of claim 2 with a nucleic acid molecule that confers male sterility. ### Claim at position 11 (depends on 10) 11: A male sterile lettuce plant produced by the method of claim 10. ### Claim at position 12 (depends on 2) 12: A method for producing an herbicide resistant lettuce plant wherein the method comprises transforming the lettuce plant of claim 2 with a transgene, wherein the transgene confers resistance to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile. ### Claim at position 13 (depends on 12) 13: An herbicide resistant lettuce plant produced by the method of claim 12. ### Claim at position 14 (depends on 2) 14: A method of producing an insect resistant lettuce plant wherein the method comprises transforming the lettuce plant of claim 2 with a transgene that confers insect resistance. ### Claim at position 15 (depends on 14) 15: An insect resistant lettuce plant produced by the method of claim 14. ### Claim at position 16 (depends on 15) 16: The lettuce plant of claim 15 wherein the transgene encodes a Bacillus thuringiensis endotoxin. ### Claim at position 17 (depends on 2) 17: A method of producing a disease resistant lettuce plant wherein the method comprises transforming the lettuce plant of claim 2 with a transgene that confers disease resistance. ### Claim at position 18 (depends on 17) 18: A disease resistant lettuce plant produced by the method of claim 17. ### Claim at position 19 (depends on 2) 19: A method of producing a lettuce plant with a value-added trait, wherein the method comprises transforming the lettuce plant of claim 2 with a transgene encoding a protein selected from the group consisting of a ferritin, a nitrate reductase, and a monellin. ### Claim at position 20 (depends on 19) 20: A lettuce plant with a value-added trait produced by the method of claim 19. ### Claim at position 21 21: A lettuce plant, or a part thereof, having all of the physiological and morphological characteristics of lettuce cultivar CVX-35, wherein a representative sample of seed of the cultivar was deposited under ATCC Accession No. PTA-______. ### Claim at position 22 22: A method of introducing a desired trait into lettuce cultivar CVX-35 wherein the method comprises: a) crossing a CVX-35 plant grown from CVX-35 seed, wherein a representative sample of seed was deposited under ATCC Accession No. PTA-______, with a plant of another lettuce cultivar that comprises a desired trait to produce F1 progeny plants, wherein the desired trait is selected from the group consisting of male sterility, herbicide resistance, insect resistance, and resistance to bacterial disease, fungal disease, or viral disease; b) selecting one or more progeny plants that have the desired trait to produce selected progeny plants; c) crossing the selected progeny plants with the CVX-35 plants to produce backcross progeny plants; d) selecting for backcross progeny plants that have the desired trait and all of the physiological and morphological characteristics of lettuce cultivar CVX-35 listed in Table 1 to produce selected backcross progeny plants; and e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and all of the physiological and morphological characteristics of lettuce cultivar CVX-35 listed in Table 1. ### Claim at position 23 (depends on 22) 23: A lettuce plant produced by the method of claim 22, wherein the plant has the desired trait and all of the physiological and morphological characteristics of lettuce cultivar CVX-35 listed in Table 1. ### Claim at position 24 (depends on 23) 24: The lettuce plant of claim 23, wherein the desired trait is herbicide resistance and the resistance is conferred to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile. ### Claim at position 25 (depends on 23) 25: The lettuce plant of claim 23, wherein the desired trait is insect resistance and the insect resistance is conferred by a transgene encoding a Bacillus thuringiensis endotoxin. ### Claim at position 26 (depends on 23) 26: The lettuce plant of claim 23, wherein the desired trait is male sterility and the trait is conferred by a nucleic acid molecule.