# US 2017/0280665 A1 · Inbred corn line lk1

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- Number: US 2017/0280665 A1
- Document ID: US20170280665A1
- Status: Published application
- Kind: A1
- Application number: 15/624,236
- Filed: 2017-06-15
- Published: 2017-10-05
- Earliest claimed: 2014-03-18
- Assignees: Limagrain Europe S.A.; Kws Saat Ae
- Applicants: Limagrain Europe, S.A.; Kws Saat Ae
- Inventors: Steven H. SCHUETZ; Michael R. BUENDGEN; Robenzon LORENZANA; Freeman WHITEHEAD; Keith WILLIAMS; Craig KLEINSCHMIDT; Tim CUPKA
- Web page: https://patentgrep.com/patent/US20170280665A1

## CPC

- A01H 6/4684: {Zea mays [maize]}
- A01H 5/10: Seeds
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

Inbred corn lines, designated BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65, are disclosed. The invention relates to the seeds of inbred corn lines BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65, to the plants and plant parts of inbred corn lines BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 and to methods for producing a corn plant, either inbred or hybrid, by crossing inbred corn lines BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 with itself or another corn line. The invention also relates to products produced from the seeds, plants, or parts thereof, of inbred corn lines BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 and/or of the hybrids produced using the inbred as a parent. The invention further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other inbred corn lines derived from inbred corn lines. BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65.

## Claims

### Claim 1

A seed of inbred corn line designated BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65, wherein a representative sample of seed of said line was deposited under ATCC Accession No. PTA-______.

### Claim 2 (depends on 1)

A corn plant, or a part thereof, produced by growing the seed of claim 1.

### Claim 3

A corn plant, or a part thereof, having all the physiological and morphological characteristics of inbred line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65, wherein a representative sample of seed of said line was deposited under ATCC Accession No. PTA-______.

### Claim 4 (depends on 2)

A tissue culture of cells produced from the plant of claim 2.

### Claim 5 (depends on 4)

A corn plant regenerated from the tissue culture of claim 4, wherein the regenerated plant has all the morphological and physiological characteristics of inbred line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65, wherein a representative sample of seed of said line was deposited under ATCC Accession No. PTA-______.

### Claim 6 (depends on 2)

A method for producing a hybrid corn seed wherein the method comprises crossing the plant of claim 2 with a different corn plant and harvesting the resultant hybrid corn seed.

### Claim 7 (depends on 6)

A hybrid corn seed produced by the method of claim 6.

### Claim 8 (depends on 7)

A hybrid corn plant, or part thereof, produced by growing the seed of claim 7.

### Claim 9

A method for producing inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65, wherein a representative sample of seed of said line was deposited under ATCC Accession No. PTA-______, wherein the method comprises:
a) planting a collection of seeds comprising seed of a hybrid, one of whose parents is inbred line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65, said collection also comprising seed of said inbred;
b) growing plants from said collection of seeds;
c) identifying the plants having the physiological and morphological characteristics of inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 as inbred parent plants;
d) controlling pollination of said inbred parent plants in a manner which preserves the homozygosity of said inbred parent plant; and
e) harvesting the resultant seed and thereby producing an inbred corn line having all of the physiological and morphological characteristics of inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65.

### Claim 10 (depends on 9)

The method of claim 9 wherein step (c) comprises identifying plants with decreased vigor compared to the other plants grown from the collection of seeds.

### Claim 11 (depends on 2)

A method for producing a corn plant that contains in its genetic material one or more transgenes, wherein the method comprises crossing the corn plant of claim 2 with either a second plant of another corn line which contains a transgene or a transformed corn plant of the inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65, so that the genetic material of the progeny plant that results from the cross contains the transgene(s) operably linked to a regulatory element and wherein the transgene is selected from the group consisting of male sterility, male fertility, herbicide resistance, insect resistance, disease resistance, water stress tolerance, and increased digestibility.

### Claim 12 (depends on 11)

A corn plant, or a part thereof, produced by the method of claim 11.

### Claim 13 (depends on 12)

The corn plant of claim 12, wherein the transgene confers resistance to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile.

### Claim 14 (depends on 12)

The corn plant of claim 12, wherein the transgene encodes a Bacillus thuringiensis protein.

### Claim 15 (depends on 12)

The corn plant of claim 12, wherein the transgene confers disease resistance.

### Claim 16 (depends on 12)

The corn plant of claim 12, wherein the transgene confers water stress tolerance.

### Claim 17 (depends on 12)

The corn plant of claim 12, where in the transgene confers increased digestibility.

### Claim 18 (depends on 12)

A method for producing a hybrid corn seed wherein the method comprises crossing the plant of claim 12 with a different corn plant and harvesting the resultant hybrid corn seed.

### Claim 19 (depends on 2)

A method of producing a corn plant with increased waxy starch or increased amylose starch wherein the method comprises transforming the corn plant of claim 2 with a transgene that modifies waxy starch or amylose starch metabolism, thereby producing a corn plant with increased waxy starch or amylose starch metabolism.

### Claim 20 (depends on 19)

A corn plant produced by the method of claim 19.

### Claim 21

A method of introducing one or more desired'traits into inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 wherein the method comprises:
a) crossing the inbred line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 plants grown from the inbred line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 seed, wherein a representative sample of seed of said line was deposited under ATCC Accession No. PTA-______, with plants of another corn line that comprise one or more desired traits to produce progeny plants, wherein the one or more desired traits are selected from the group consisting of male sterility, male fertility, herbicide resistance, insect resistance, disease resistance, waxy starch, water stress tolerance, increased amylose starch and increased digestibility;
b) selecting progeny plants that have the one or more desired traits to produce selected progeny plants;
c) crossing the selected progeny plants with the inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 plants to produce backcross progeny plants;
d) selecting for backcross progeny plants that have the one or more desired traits and physiological and morphological characteristics of inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 listed in Table 1A to Table 1S to produce selected backcross progeny plants; and
e) repeating steps (c) and (d) one or more times in succession to produce selected second or higher backcross progeny plants that comprise the desired one or more traits and all of the physiological and morphological characteristics of inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5,LK1, and MM65 as listed in Table 1A to Table 1S.

### Claim 22 (depends on 21)

A corn plant produced by the method of claim 21, wherein the plant has the one or more desired traits and all of the physiological and morphological characteristics of inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 as listed in Table 1A to Table 1S.

### Claim 23 (depends on 2)

A method for producing inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 seed, wherein a representative sample of seed of said line was deposited under ATCC Accession No. PTA-______, wherein the method comprises crossing a first inbred parent corn plant with a second inbred parent corn plant and harvesting the resultant corn seed, wherein both said first and second inbred corn plant are the corn plant of claim 2.

### Claim 24 (depends on 1)

A method for producing inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 seed, wherein a representative sample of seed of said line was deposited under ATCC Accession No. PTA-______, wherein the method comprises:
a) planting the inbred corn seed of claim 1;
b) growing a plant from said seed;
c) controlling pollination in a manner that the pollen produced by the grown plant pollinates the ovules produced by the grown plant; and
d) harvesting the resultant seed and thereby producing an inbred corn line having all of the physiological and morphological characteristics of inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65.

### Claim 25 (depends on 2)

A method for producing a corn seed that contains in its genetic material one or more transgenes, wherein the method comprises crossing the corn plant of claim 2 with either a second plant of another corn line which contains one or more transgenes or a transformed corn plant of the inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65, wherein the transgene(s) is operably linked to a regulatory element and wherein the transgene is selected from the group consisting of male sterility, male fertility, herbicide resistance, insect resistance, disease resistance, water stress tolerance, and increased digestibility; and harvesting the resultant seed.

### Claim 26 (depends on 25)

A corn seed, or a part thereof, produced by the method of claim 25.

### Claim 27 (depends on 22)

A method for producing a hybrid corn seed wherein the method comprises crossing the plant of claim 22 with a different corn plant and harvesting the resultant hybrid corn seed.

### Claim 28 (depends on 27)

A hybrid corn seed produced by the method of claim 27.

### Claim 29 (depends on 1, 2)

A corn product produced using the inbred seed of claim 1 or produced using the inbred corn plant, or part thereof, of claim 2.

### Claim 30 (depends on 30)

The corn product of claim 30, wherein the corn product is selected from the group consisting of corn meal, corn flour, corn starch, corn syrup, corn sweetener and corn oil.

### Claim 31 (depends on 7, 8)

A corn product produced using the hybrid seed of claim 7 or produced using the hybrid corn plant, or part thereof, of claim 8.

### Claim 32 (depends on 32)

The corn product of claim 32, wherein the corn product is selected from the group consisting of corn meal, corn flour, corn starch, corn syrup, corn sweetener and corn oil.