Xyloglucan Endotransglycosylase variants and Polynucleotides Encoding Same
AssigneeNovozymes A/S
InventorDaniel Held
Life of this patent
Estimating the term…
Abstract
The present invention relates to xyloglucan endotransglycosylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims20
A xyloglucan endotransglycosylase variant, comprising a substitution at one or more positions corresponding to positions 10, 30, 40, 51, 53, 60, 99, 102, 117, 130, 136, 157, 162, 175, 183, 254, and 280 of the full-length polypeptide of SEQ ID NO:
- 2, wherein the variant has xyloglucan endotransglycosylase activity and wherein the variant has at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, sequence identity to the mature polypeptide sequence of SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, or 50.
A method for obtaining a xyloglucan endotransglycosylase variant, comprising introducing into a parent xyloglucan endotransglycosylase a substitution at one or more positions corresponding to positions 10, 30, 40, 51, 53, 60, 99, 102, 117, 130, 136, 157, 162, 175, 183, 254, and 280 of the full-length polypeptide of SEQ ID NO:
- 2, wherein the variant has xyloglucan endotransglycosylase activity;
- and optionally recovering the variant.
A method of increasing the expression yield of a xyloglucan endotransglycosylase, comprising introducing into a parent xyloglucan endotransglycosylase a substitution at one or more positions corresponding to positions 10, 30, 40, 51, 53, 60, 99, 102, 117, 130, 136, 157, 162, 175, 183, 254, and 280 of the full-length polypeptide of SEQ ID NO:
- 2 to produce a variant, wherein the expression yield of the variant is increased relative to the parent;
- and optionally recovering the variant.
(canceled)
Dependencies are inferred from the claim text.