Sortase-modified molecules and uses thereof
AssigneeResearch Development Foundation
InventorsXinjian LIN, Xiying SHANG, Stephen B. HOWELL
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Abstract
Cell-targeted cytotoxic agents, including sortase serine protease constructs, are provided. Such compounds can be used in methods for targeted cell killing such as for treatment cell of proliferative diseases (e.g., cancer). In some aspects, recombinant sortase serine proteases, such as Granzyme B polypeptides, are provided that exhibit improved stability and cell toxicity.
Claims29
A compound comprising a cytotoxic agent conjugated to a sortase recognition sequence.
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A recombinant fusion polypeptide comprising:
- (a) a recombinant cytotoxic polypeptide;
- (b) a sortase linker;
- and
- (c) a cell-targeting polypeptide,
- wherein the sortase linker is positioned between the cytotoxic polypeptide and the cell-targeting polypeptide.
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Dependencies are inferred from the claim text.