Enzymes for removing sulfurous compounds in downhole fluids
AssigneeBaker Hughes Incorporated
InventorsPrasad Dhulipala, Jerry Weers, Soma Chakraborty, Marilyn Blaschke
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Abstract
Cysteine synthase enzymes (e.g. O-Acetyl-L-Serine Sulfhydrylase enzymes) may be used in combination with sulfide quinone reductase enzymes in additive compositions, fluid compositions, and methods for decreasing or removing hydrogen sulfide from recovered downhole fluids and/or the subterranean reservoir wellbore from which the downhole fluid was recovered. The fluid composition may include at least one cysteine synthase enzyme with at least one sulfide quinone reductase, and a base fluid, such as a water-based fluid, an organic-based fluid, and combinations thereof.
Claims21
An additive composition for a base fluid selected from the group consisting of a drilling fluid, a completion fluid, a production fluid, a servicing fluid, an injection fluid, a refinery fluid, and combinations thereof;
- wherein the additive composition comprises at least one cysteine synthase enzyme and at least one sulfide quinone reductase enzyme;
- wherein the at least one cysteine synthase enzyme is at least 75% homologous to the cDNA sequence of SEQ ID NO:1;
- and wherein the at least one sulfide quinone reductase is at least 75% homologous to the cDNA sequence of SEQ ID NO:2.
A fluid composition comprising:
- a base fluid selected from the group consisting of drilling fluids, servicing fluids, production fluids, completion fluids, injection fluids, refinery fluids, and combinations thereof;
- at least one cysteine synthase enzyme that is at least 75% homologous to the cDNA sequence of SEQ ID NO:1;
- and
- at least one sulfide quinone reductase enzyme is at least 75% homologous to the cDNA sequence of SEQ ID NO:2.
A method comprising:
- contacting at least one fluid with at least one cysteine synthase enzyme that is at least 75% homologous to the cDNA sequence of SEQ ID NO:1 in an effective concentration to decrease an amount of sulfur-containing compounds within the at least one fluid;
- wherein the at least one fluid is selected from the group consisting of drilling fluids, servicing fluids, production fluids, completion fluids, injection fluids, refinery fluids, and combinations thereof;
- contacting the at least one fluid with at least one sulfide quinone reductase enzyme that is at least 75% homologous to the cDNA sequence of SEQ ID NO:2 in an effective concentration to decrease an amount of sulfur-containing compounds within the at least one fluid;
- decreasing an amount of sulfur-containing compounds in the at least one fluid.
Dependencies are inferred from the claim text.