Analyzing a metabolite level in a sample
AssigneePurdue Research Foundation
InventorsRobert Graham Cooks, Kevin Scott Kerian, Alan Keith Jarmusch, Ahmed Mohamed Hamid, Livia Schiavinato Eberlin
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Abstract
The invention generally relates to methods for analyzing a metabolite level in a sample. In certain embodiments, methods of the invention may involve obtaining a sample, analyzing the sample using a mass spectrometry technique to determine a level of at least one metabolite in the sample, and correlating the metabolite level with an originating source of the sample, thereby analyzing the sample.
Claims13
1-20. (canceled)
A method for determining a tumor margin, the method comprising:
- depositing a tissue sample onto a substrate, wherein the tissue sample comprises at least one metabolite indicative of a cancer and a plurality of reference molecules that are not associated with the cancer;
- conducting a desorption electrospray ionization (DESI) technique on one or more locations of the tissue sample in order to desorb the at least one metabolite and the plurality of reference molecules from the one or more locations of the tissue sample and direct the at least one metabolite and the plurality of reference molecules into a mass spectrometer to thereby generate a mass spectral signal of the at least one metabolite and a plurality of reference mass spectral signals at the one or more locations;
- normalizing the mass spectral signal of the at least one metabolite at the one or more locations to one or more of reference mass spectral signals from the tissue sample in order to obtain a relative abundance of the at least one metabolite at the one or more locations within the tissue section;
- and
- defining one or more tumor margins based on the relative abundance of the at least one metabolite at the one or more locations of the tissue sample.
The method according to claim 1, wherein the plurality of reference molecules are lipids.
The method according to claim 36, wherein the histochemistry analysis technique is H&E staining or immunohistochemistry.
Dependencies are inferred from the claim text.