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US 2017/0248606 A1Published application
Apparatuses, methods, computer program products, and kits for hi-throughput glycan analysis
AssigneeLife Technologies Corporation
InventorPeter Slade
Life of this patent
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Abstract
An apparatus for glycan analysis is disclosed. The apparatus includes a plurality of loading wells adapted to receive a plurality of samples; a plurality of capillaries arranged in correspondence with the loading wells, each of the capillaries including a first portion including a stacking gel and a second portion including a resolving gel; and a plurality of eluting wells arranged in correspondence with the capillaries and adapted to receive a portion of the samples having traversed the capillaries.
Claims21
-121. (canceled)
A method for glycan analysis, the method comprising:
- loading a plurality of samples into a plurality of loading wells, wherein the plurality of samples include glycoproteins;
- denaturing the glycoproteins to form denatured glycoproteins;
- cleaving one or more glycans from the denatured glycoproteins;
- labeling the cleaved glycans with one or more fluorescent labels to form labeled glycans;
- migrating the labeled glycans across a plurality of capillaries;
- exciting the one or more fluorescent labels of the labeled glycans to generate fluorescence;
- detecting the fluorescence;
- and
- analyzing the labeled glycans based on the fluorescence.
6 more elements
Dependencies are inferred from the claim text.