Methods for diagnosis, prognosis and methods of treatment

A method of drug screening, said method comprising:

• A] classifying one or more hematopoietic cells associated with acute leukemia, myelodysplastic syndrome or myeloproliferative neoplasms in said individual by a method comprising:
• a) subjecting a cell population comprising said one or more hematopoietic cells from said individual to a test compound and a plurality of modulators in a plurality of cultures,
• b) characterizing a plurality of pathways in one or more cells from said plurality of cultures by determining an activation level of at least one activatable element within said plurality of pathways, wherein
• i) at least one of said plurality of pathways being characterized in at least one of said plurality of cultures is an apoptosis or a DNA damage pathway,
• ii) said plurality of modulators activate or inhibit one or more of said plurality of pathways being characterized, and
• c) classifying said one or more hematopoietic cells based on said pathways characterization;
• and
• B] making a decision regarding said test compound and its therapeutic potential for treatment of acute leukemia, myelodysplastic syndrome or myeloproliferative neoplasms, wherein said decision is based on said classification of said one or more hematopoietic cells.