Sensing sensor and sensing method

A sensing sensor comprising:

• a wiring board configured to include a connection terminal and a depressed portion, the connection terminal being for connection to a measuring device for measuring an oscillation frequency, the depressed portion being formed on one surface side of the wiring board;
• a piezoelectric resonator configured to include a piezoelectric piece and an excitation electrode on the piezoelectric piece, the piezoelectric resonator being secured to the wiring board such that the piezoelectric resonator covers the depressed portion and a vibrating region is opposed to the depressed portion, the excitation electrode being electrically connected to the connection terminal, the piezoelectric resonator having one surface side on which an adsorbing film constituted of biomolecules for adsorbing a sensing object in a sample solution is formed;
• a gel-like protective agent having a high viscosity, disposed to cover a surface of the adsorbing film for suppressing an inactivation of the biomolecules;
• a channel forming member disposed to cover a region of the one surface side of the wiring board including the piezoelectric resonator, the channel for icing member including an injection port of the sample solution;
• and
• a flow passage disposed between the wiring board and the channel forming member and configured to allow the sample solution supplied to the injection port to flow from one end side to another end side on the one surface side of the piezoelectric resonator.