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US 2017/0248589 A1Published application
Method of measuring interaction between molecules
AssigneePharmadiagnostics NV
InventorsMeike Roskamp, Vanessa Bonnard, Sylviane Boucharens
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Abstract
The present invention relates to methods of measuring interaction between a first and a second molecule, for example a protein and an antibody, by conjugation of one of these molecules with nanoparticles, and measuring the interaction between the first and second molecule via changes in the optical properties of the nanoparticles. The present invention further relates to methods of coating nanoparticles.
Claims17
A method of coating a metal nanoparticle with a compound, comprising:
- i) providing a liquid composition comprising metal nanoparticles at least partially coated with cetyl trimethyl ammonium bromide (CTAB);
- ii) adding a thiol-polyethylene glycol to said composition of step i), thereby obtaining a liquid composition comprising metal nanoparticles coated with thiol-polyethylene glycol;
- iii) purification of said composition obtained in step ii) by separating said nanoparticles from free thiol-polyethylene glycol and free CTAB;
- iv) adding a mercaptocarboxylic acid to said composition obtained in step iii), thereby obtaining a liquid composition comprising metal nanoparticles coated with mercaptocarboxylic acid, and
- v) contacting said metal particles obtained in step iv) with said compound.
2 more elements
Dependencies are inferred from the claim text.