Polymeric Dye Specific Binding Members and Methods of Making and Using the Same
AssigneeBecton, Dickinson and Company
InventorsJames Ghadiali, Jody Martin
Life of this patent
Estimating the term…
Abstract
Proteinaceous specific binding members that specifically bind to a polymeric dye are provided. Also provided are methods of using the specific binding members, e.g., in separating a polymeric dye-labeled cell from a sample, in analyte detection, etc., as described herein. Kits and systems for practicing the subject methods are also provided.
Claims15
A proteinaceous specific binding member that specifically binds to a polymeric dye.
The specific binding member according to any of the preceding claims, wherein the polymeric dye is a polymeric tandem dye.
The specific binding member according to any of the preceding claims, wherein the specific binding member is selected from the group consisting of an antibody, a Fab fragment, a F(ab′)2 fragment, a scFv, a diabody, or a triabody.
The specific binding member according to any of the preceding claims, wherein the specific binding member is support bound.
A method comprising contacting a sample with a specific binding member according to any of the preceding claims.
A kit comprising:
- a proteinaceous specific binding member that specifically binds to a polymeric dye;
- and
- one or more components selected from the group consisting of a polymeric dye, a polymeric tandem dye, a polymeric dye-specific binding member conjugate, a cell, a support, an biocompatible aqueous elution buffer, and instructions for use.
A flow cytometric system, comprising:
- a flow cytometer comprising a flow path;
- a composition in the flow path, wherein the composition comprises:
- a cell-containing biological sample;
- and
- a polymeric dye-specific binding member conjugate that specifically binds a target cell.
Dependencies are inferred from the claim text.