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US 2017/0248582 A1Published application
Mixed Spheroids of Melanocytes and Keratinocytes
AssigneesSyntivia, Centre Hospitalier Universitaire De Toulouse, Universite Paul Sabatier Toulouse III, Centre National De La Recherche Scientifique
InventorsMarine Norlund, Bernard Ducommun, Philippe Bedos, Valérie Lobjois
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Abstract
The present invention concerns the development of a model to evaluate active substances targeting the epidermis. It more particularly relates to the preparation of mixed spheroids of melanocytes and keratinocytes reproducing cell interactions occurring in the epidermis, to the spheroids as such and to the uses thereof.
Claims17
Method for preparing mixed spheroids of keratinocytes and melanocytes, characterized in that it comprises:
- (a) suspending keratinocytes with melanocytes in a culture medium supplemented with a preparation of extracellular matrix proteins at a concentration of between 1 and 95% by volume/volume and an amount of 10 mM or less of a salt selected from among calcium, manganese or magnesium;
- (b) forming spheroids from the mixture obtained at step (a);
- and
- (c) incubating the spheroids obtained at step (b).
1 more element
Dependencies are inferred from the claim text.