Refractive index-based detection for liquid chromatography using a microring resonator array
AssigneeThe Board of Trustees of the University of Illinois
InventorsRyan C. BAILEY, James H. WADE
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Abstract
A liquid chromatograph comprising a column coupled to a microring resonator array and methods of using the same are disclosed. The microring resonator array measures the bulk refractive index of the mobile phase and any sample injected onto and separated in the column. While carrying out the methods, the composition of a mobile phase passing through the chromatography column may remain substantially constant (isocratic elution) or it may vary (gradient elution). One or more microrings may comprise a covering to act as a thermal control. In addition, the sensor surface may be modified with some type of capture agent that can interact with one or more components in the sample.
Claims25
A liquid chromatography system comprising:
- a chromatography column operatively coupled to a microring resonator array, wherein the microring resonator array is in fluid communication with the chromatography column.
A liquid chromatography method comprising:
- pumping a mobile phase through a conduit into a chromatography column;
- injecting a sample comprising one or more components into the mobile phase, the sample being separated into the one or more components in the chromatography column;
- allowing the mobile phase and the one or more components to exit the chromatography column during an elution period and enter a flow cell of a detector, wherein the detector comprises a microring resonator array;
- and
- analyzing the one or more components with the microring resonator array.
A liquid chromatography method comprising:
- pumping a mobile phase through a conduit into a chromatography column;
- injecting a sample comprising one or more components into the mobile phase, the sample being separated into the one or more components in the chromatography column;
- allowing the mobile phase and the one or more components to exit the chromatography column during an elution period and enter a flow cell of a detector;
- and
- measuring a bulk refractive index of the mobile phase and the one or more components, wherein a composition of the mobile phase changes during the elution period.
Dependencies are inferred from the claim text.