Dynamic Orthogonal Analysis Method

A method for providing a differential mobility spectrometry (DMS) precursor ion survey scan, comprising:

• instructing an ion source configured to receive a sample to ionize the sample using a processor;
• instructing a differential mobility spectrometry (DMS) device configured to receive ions from the ion source to separate precursor ions received from the ion source and transmit precursor ions using two or more compensation voltages (CoVs) using the processor;
• instructing a mass analyzer configured to receive transmitted precursor ions from the DMS device to measure the m/z intensities of the transmitted precursor ions across an m/z range at each CoV of the two or more CoVs using the processor;
• and
• storing the measured m/z intensities of the transmitted precursor ions received from the mass analyzer as a function of m/z value and CoV, producing a two-dimensional mapping of m/z intensities of the precursor ions of the sample using the processor.

A computer program product, comprising a non-transitory and tangible computer-readable storage medium whose contents include a program with instructions being executed on a processor so as to perform a method for providing a differential mobility spectrometry (DMS) precursor ion survey scan, the method comprising:

• providing a system, wherein the system comprises one or more distinct software modules, and wherein the distinct software modules comprise a control module;
• instructing an ion source configured to receive a sample to ionize the sample using the control module;
• instructing a differential mobility spectrometry (DMS) device configured to receive ions from the ion source to separate precursor ions received from the ion source and transmit precursor ions using two or more compensation voltages (CoVs) using the control module;
• instructing a mass analyzer configured to receive transmitted precursor ions from the DMS device to measure the m/z intensities of the transmitted precursor ions across an m/z range at each CoV of the two or more CoVs using the control module;
• and
• storing the measured m/z intensities of the transmitted precursor ions received from the mass analyzer as a function of m/z value and CoV, producing a two-dimensional mapping of m/z intensities of the precursor ions of the sample using the control module.