Methods, computer-readable media, and systems for assessing samples and wounds, predicting whether a wound will heal, and monitoring effectiveness of a treatment

A method of assessing a wound, the method comprising:

• extracting RNA from debrided wound tissue;
• measuring expression of one or more genes within the RNA;
• and
• calculating a ratio of M1 macrophages to M2 macrophages based on the measured gene expression.

A method of monitoring effectiveness of a treatment of a non-healing wound or a tumor, the method comprising:

• administering to a patient a therapeutic agent designed to treat a non-healing wound or a tumor;
• obtaining a first measurement of a first macrophage phenotype population within a first sample obtained from the non-healing wound or the tumor;
• obtaining a second measurement of second macrophage phenotype population from the non-healing wound or the tumor, wherein the second measurement of the second macrophage phenotype population is either:
• a different macrophage phenotype obtained from the first sample or the tumor;
• or
• the same macrophage phenotype obtained from a second, later sample from the non-healing wound or the tumor;
• comparing the first measurement to the second measurement;
• and
• assessing whether the treatment of the non-healing wound or the tumor is effective based on a result of comparing the measurements.