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US 2017/0247757 A1Published application
Methods and systems for processing polynucleotides
Assignee10x Genomics, Inc.
InventorsBenjamin Hindson, Serge Saxonov, Kevin Ness, Paul Hardenbol, Michael Schnall-Levin, Mirna Jarosz
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Abstract
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
Claims30
A method for nucleic acid sequence analysis, comprising:
- (a) providing a plurality of partitions, a plurality of polynucleotide molecules and a plurality of barcode molecules, wherein said plurality of barcode molecules comprise a plurality of barcode sequences, wherein at least 1,000 partitions of said plurality of partitions each comprise (i) a polynucleotide molecule from said plurality of polynucleotide molecules, and (ii) a barcode molecule from said plurality of barcode molecules, wherein said plurality of barcode sequences are different across said at least 1,000 partitions;
- (b) using said plurality of polynucleotide molecules and said plurality of barcode molecules in said at least 1,000 partitions to generate a plurality of barcoded polynucleotide molecules, which plurality of barcoded polynucleotide molecules comprises said plurality of barcode sequences that are different across said at least 1,000 partitions;
- and
- (c) determining sequences of said plurality of barcoded polynucleotide molecules.
1 more element
Dependencies are inferred from the claim text.