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US 2017/0247753 A1Published application
Method of Normalizing Biological Samples
AssigneeSiemens Healthcare Diagnostics Inc.
InventorsThomas Battersby, Adrian Nielsen Fehr, Minxue Zheng
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Abstract
The present disclosure relates to normalization of biological samples, particularly samples comprising nucleic acids to be sequenced. The normalization protocols described herein may be utilized across multiple samples to cap total stoichiometric input and minimize variations in transcript abundance on a per-sample basis in a multiplexed fashion to dramatically increase the accuracy, capacity and efficiency of nucleic acid sequencing.
Claims1
A kit, comprising:
- a plurality of oligonucleotide capture tags capable of binding target polynucleotides, wherein each capture tag comprises a unique identifying and is at least partially single stranded;
- an oligonucleotide capture moiety capable of binding the capture tag in solution, wherein each capture moiety comprises a first part that binds to the capture tags and a second part that binds to probes attached to the solid support;
- and
- a solid support comprising universal oligonucleotide probes capable of binding the capture moiety, wherein the probes comprise an identical nucleotide sequence capable of hybridizing to a corresponding sequence found in each capture moiety.
1 more element
Dependencies are inferred from the claim text.