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US 2017/0247752 A1Published application
Cooperative primers, probes, and applications thereof
AssigneeDNA Logix, Inc.
InventorBrent C. Satterfield
Life of this patent
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Abstract
Disclosed are compositions and a method relating to amplifying and detecting nucleic acids.
Claims20
A cooperative nucleic acid molecule comprising:
- a. a first nucleic acid sequence, wherein the first nucleic acid sequence is complementary to a first region of a target nucleic acid, and wherein the first nucleic acid is extendable on the 3′ end;
- b. a second nucleic acid sequence, wherein the second nucleic acid sequence is complementary to a second region of the target nucleic acid, such that in the presence of the target nucleic acid it hybridizes to the target nucleic acid downstream from the 3′ end of the first nucleic acid sequence;
- c. a linker connecting said first and second nucleic acid sequences in a manner that allows both the said first and second nucleic acid sequences to hybridize to the target at the same time.
A method for synthesizing a nucleic acid, the method comprising:
- a. contacting a target nucleic acid with
- b. a cooperative nucleic acid molecule comprising:
- i. a first nucleic acid sequence, wherein the first nucleic acid sequence is complementary to a first region of a target nucleic acid, and wherein the first nucleic acid is extendable on the 3′ end;
- ii. a second nucleic acid sequence, wherein the second nucleic acid sequence is complementary to a second region of the target nucleic acid, such that it hybridizes to the target nucleic acid downstream from the 3′ end of the first nucleic acid sequence;
- iii. a linker connecting said first and second nucleic acid sequences in a manner that allows both the said first and second nucleic acid sequences to hybridize to the target at the same time;
- c. and providing conditions appropriate for nucleic acid synthesis, thereby synthesizing a nucleic acid.
3 more elements
A method for detecting a target nucleic acid, the method comprising:
- a. contacting a sample containing the target nucleic acid with
- b. a cooperative nucleic acid molecule comprising:
- i. a first nucleic acid sequence, wherein the first nucleic acid sequence is complementary to a first region of a target nucleic acid, and wherein the first nucleic acid is extendable on the 3′ end;
- ii. a second nucleic acid sequence, wherein the second nucleic acid sequence is complementary to a second region of the target nucleic acid, such that it hybridizes to the target nucleic acid downstream from the 3′ end of the first nucleic acid sequence;
- iii. a linker connecting said first and second nucleic acid sequences in a manner that allows both the said first and second nucleic acid sequences to hybridize to the target at the same time;
- c. and detecting the target analyte.
3 more elements
A method for amplifying a target nucleic acid, the method comprising:
- a) providing the cooperative nucleic acid molecule of claim 1;
- b) providing a target nucleic acid;
- and
- c) amplifying the target nucleic acid under appropriate conditions for amplification
- The method of claim 14, wherein the first nucleic acid sequence is a primer.
2 more elements
Dependencies are inferred from the claim text.