Apparatus and methods for detecting multiple labelled biopolymers
AssigneeMicroscale Devices LLC
InventorJohn A. Humphrey
Life of this patent
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Abstract
The disclosure is directed toward instrumentation/processes for generating multiple datapoints from a sample of multiple comingled labeled biopolymers. In one embodiment, it relates the description of optical approaches used to integrate the samples, the optical approaches used to alter the light path along the “Z” axis perpendicular to the “X” and “Y” axes of a plate containing multiple samples in multiple sample wells and thereby provide greater functionality and the integration of these approaches with purification methods. Alternatively, it provides methods of analyzing beads with multiple labeled nucleic acids attached.
Claims19
A system for analyzing multiple labeled nucleic acids in a sample, each labeled nucleic acids being labeled by a covalent linkage with at least one synthetic fluorescent molecule capable of generating emitted light at one or more light frequencies in response to an exciting light, the system comprising:
- a light source configured to produce an exciting light;
- an excitation filter;
- a dichroic mirror capable of directing the exciting light to the sample;
- a tunable emitted light filter capable of filtering the plurality of emitted light frequencies from the sample;
- and a detector capable of detecting the plurality of emitted light frequencies from the multiple labeled nucleic acids in the sample wherein the tunable emitted light filter is at about a 0 to 45 degree angle or a 135 to 180 degree angle to the directed exciting light and the sample.
A system for analyzing multiple labeled nucleic acids in a sample, the labeled nucleic acids being capable of generating emitted light at a plurality of light frequencies in response to an exciting light, the system comprising:
- a light source configured to produce an exciting light;
- an excitation filter;
- a multi-part prism capable of directing the exciting light to the sample;
- a tunable emitted light filter capable of filtering the plurality of emitted light frequencies from the sample;
- and a detector capable of detecting the plurality of emitted light frequencies from the multiple labeled nucleic acids in the sample.
A method of analyzing multiple labeled nucleic acids in a sample, the labeled nucleic acids being capable of generating a plurality of emitted light frequencies in response to an exciting light, the method comprising:
- producing an exciting light from a light source;
- directing the light source through an excitation filter;
- directing the filtered excitation light through a dichroic mirror capable of directing the exciting light to the sample;
- generating the plurality of emitted light frequencies from the sample and directing the emitted light to a tunable emitted light filter;
- directing the filtered emitted light to a detector capable of detecting plurality of emitted light frequencies from the multiple labeled nucleic acids in the sample.
Dependencies are inferred from the claim text.