Diagnostic Methods and Markers for Bacterial Vaginosis
AssigneeLaboratory Corporation of America Holdings
InventorsCharles Paul Cartwright, Bryndon Denae Lembke, Kalpana Ramachandran
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Abstract
A method of diagnosing bacterial vaginosis in a woman, which involves determining an amount of each of more than one BV-associated bacterium in a vaginal sample obtained from the female and assessing a BV status of the female based on the amount of each of the more than one BV-associated bacterium in the sample
Claims27
A method of detecting positive marker bacterial vaginosis (BV)-associated bacteria in a female, comprising performing on a sample obtained from the female a quantitative or a semi-quantitative polymerase chain reaction (PCR) assay to detect amounts of nucleic acid sequences characteristic of the positive marker BV-associated bacteria, wherein the positive marker BV-associated bacteria comprise BVAB-2, Megasphaera-1, and at least one of Atopobium vaginae or Gardnerella vaginalis.
A method of assessing bacterial vaginosis (BV) status and treating BV in a female, comprising:
- detecting in a sample obtained from the female amounts of nucleic acid sequences characteristic of positive marker BV-associated bacteria, wherein the positive BV-associated bacteria comprise BVAB-2, Megasphaera-1 and at least one of Atopobium vaginae or Gardnerella vaginalis, and wherein the detecting comprises performing on the sample a quantitative or a semi-quantitative polymerase chain reaction (PCR) assay to detect the amounts of the nucleic acid sequences characteristic of the positive BV-associated bacteria using at least three pairs of primers specific for the positive marker BV associated bacteria;
- assessing the BV status of the female using the detected amounts of the nucleic acid sequences characteristic of the positive marker BV-associated bacteria;
- and,
- administering a BV treatment to the female patient based on the assessed BV status.
A method for assessing bacterial vaginosis (BV) status in a female, comprising performing on a sample obtained from the female a quantitative or a semi-quantitative polymerase chain reaction (PCR) assay using a kit comprising pairs of primers and other reagents for conducting the quantitative or the semi-quantitative PCR assay for detecting in the sample obtained from the female amounts of nucleic acid sequences characteristic of positive marker BV-associated bacteria, wherein the positive marker BV-associated bacteria comprise BVAB-2, Megasphaera-1 and at least one of Atopobium vaginae or Gardnerella vaginalis.
Dependencies are inferred from the claim text.