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US 2017/0247748 A1Published application
Genome methylation analysis
AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsPeter MOLLOY, Sue MITCHELL, Konsta DUESING
Life of this patent
Estimating the term…
Abstract
Provided herein are methods for identifying sites and regions within a gene or genome that are amenable to analysis of methylation. The methods disclosed herein allow the efficient identification on a genome-wide scale of target restriction sites and fragments that provide targets for subsequent analysis.
Claims18
A method for identifying a site or region of a gene or genome for analysis, the method comprising:
- (a) obtaining one or more test samples of genomic DNA and one or more reference samples of genomic DNA;
- (b) digesting said test and reference samples with a methylation-dependent or methylation-sensitive restriction enzyme;
- (c) sequencing fragments obtained from step (b);
- (d) aligning sequences obtained from said test and reference samples with a reference genome;
- (e) comparing sequences obtained from said test and reference samples;
- and
- (f) identifying from the aligned sequences a methylated or unmethylated site or region of a gene or genome for analysis.
4 more elements
A method for analysing methylation status of a site or region of a gene or genome, the method comprising:
- (a) obtaining one or more test samples of genomic DNA and one or more reference samples of genomic DNA;
- (b) digesting said test and reference samples with a methylation-dependent or methylation-sensitive restriction enzyme;
- (c) sequencing fragments obtained from step (b);
- (d) aligning sequences obtained from said test and reference samples with a reference genome;
- (e) comparing sequences obtained from said test and reference samples;
- (f) identifying from the aligned sequences a methylated or unmethylated site or region of a gene or genome for analysis;
- and
- (g) analysing the methylation status of said site or region in one or more additional samples.
5 more elements
Dependencies are inferred from the claim text.