Multiplex optical detection
AssigneeClick Diagnostics, Inc.
InventorsKimberly M. SHULTZ, Alan D. BALDWIN, Kate L. BECHTEL, Adam DE LA ZERDA, Jesus CHING, Gregory C. LONEY
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Abstract
The present disclosure provides systems and methods for the optical detection of a plurality of labeled substrates in an assay. The various aspects of the optical detection systems enable the simultaneous detection of the plurality of labeled substrates. These systems are particularly useful in the detection of nucleic acids during an amplifications reaction.
Claims30
1) An emission module comprising
- a) a plurality of detectors;
- and
- b) a plurality of emission filters each comprising a bandpass filter for receiving and separating an emission light from a sample comprising one or more detectable labels to a predetermined emission wavelength;
- wherein each bandpass filter separates the emission light to a different predetermined emission wavelength;
- and wherein each emission filter is associated with one or more of the plurality of detectors.
16) A method for detecting one or more detectable labels positioned on a substrate, the method comprising:
- a) providing a sample comprising at least one substrate hybridized to a probe comprising at least one detectable label, wherein each detectable label emits light at an emission wavelength upon excitation by an excitation light at a predetermined excitation wavelength;
- b) exciting the at least one detectable label with the excitation light provided by an optical excitation light source;
- c) directing the emitted light to a plurality of emission filters each comprising a bandpass filter;
- wherein each bandpass filter separates the emitted light to a different predetermined emission wavelength;
- and
- d) detecting the emitted light with a plurality of detectors, wherein each predetermined emission wavelength is detected by a different detector.
23) A method for monitoring a thermocycling reaction, the method comprising:
- a) providing a thermal cycler comprising
- i) a first chamber for holding fluid at a first average temperature, and
- ii) a second chamber for holding the fluid at a second average temperature, the second chamber in fluid communication with the first chamber;
- b) introducing a sample into either the first chamber or the second chamber, wherein the sample comprises or suspected of comprising a target nucleic acid molecule and one or more detectably labeled probes configured to hybridize to the target nucleic acid molecule;
- c) transferring the sample from the first chamber to the second chamber;
- and
- d) measuring a detectable signal emitting from the sample in response to a stimulus using an optical detection emission module.
Dependencies are inferred from the claim text.