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US 2017/0247744 A1Published application
Devices and methods for capturing target molecules
AssigneesHitachi Chemical Company, Ltd., Hitachi Chemical Company America, Ltd.
InventorsMasato Mitsuhashi, Taku Murakami
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Abstract
Provided herein are devices and methods for the capture or isolation of a biomarker from a biological sample. In several embodiments, the device comprises a loading region, a filter material, and a receiving region. In particular, in several embodiments, biological fluid is passed from the loading region through the filter material and into the receiving region, thereby resulting in capture or isolation of a biomarker.
Claims20
A method of isolating vesicles from biological fluid, comprising:
- (a) obtaining a biological fluid sample comprising said vesicles;
- (b) adjusting the salt concentration of the fluid sample to between about 10 mM and 900 mM;
- (c) adjusting the pH of the fluid sample to between about pH 6 and pH 9;
- and
- (d) passing said biological fluid sample through a vesicle-capture material, said vesicle-capture material comprising glass-like materials, to produce a supernatant, wherein the vesicles from said biological fluid sample are captured on or in said vesicle-capture material.
2 more elements
A method of isolating vesicles from biological fluid, comprising:
- (a) obtaining a biological fluid sample comprising said vesicles;
- (b) adjusting the salt concentration of the fluid sample to between about 10 mM and 900 mM;
- (c) adjusting the pH of the fluid sample to between about pH 6 and pH 9;
- and
- (d) passing said biological fluid sample through a vesicle-capture material adapted to capture said vesicles by van der Waals forces.
2 more elements
Dependencies are inferred from the claim text.