Methods of using acyl-coa synthetase for biosynthetic production of acyl-coas
AssigneeConagen Inc.
InventorsHui Chen, Hongxue Wang
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Abstract
A biosynthetic method of making carboxyl CoA from long-chain carboxylic acid including expressing an ACS in a cellular system, feeding a long chain carboxylic acid to the cellular system, growing the cellular system in a medium, and producing carboxyl CoA.
Claims21
A biosynthetic method of making carboxyl CoA from medium-chain carboxylic or long-chain carboxylic acid comprising:
- expressing an ACS in a cellular system;
- feeding a medium-chain carboxylic acid or a long-chain carboxylic acid or both to the cellular system;
- growing the cellular system in a medium;
- and
- producing carboxyl CoA.
A biosynthetic method of making 8-methylnonenoyl-CoA comprising:
- expressing an ACS in a cellular system;
- feeding 8-methyl-trans-6-nonenoic acid to the cellular system;
- growing the cellular system in a medium;
- and
- producing 8-methylnonenoyl-CoA.
The use of ACS1 to modulate the levels of capsaicinoids in pepper plants comprising overexpressing ACS1;
- or knocking out or knocking down ACS1.
The use of ACS1 to modulate levels of acyl-CoAs and their downstream metabolites including fatty acids comprising overexpressing ACS1 in a cellular system;
- or knocking out or knocking down ACS1 in a cellular system.
Dependencies are inferred from the claim text.