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AssigneeBASF SE
InventorsMichael BUDDE, Michael BRAUN, Juergen DAEUWEL, Peter OEDMAN, Kai-Uwe BALDENIUS, Matthias KLEINER, Stephan FREYER
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The present invention relates to means and methods for producing an amide compound from a nitrile compound with less acrylic acid as by-product using a Nitrile hydratase (NHase) and Amidase producing microorganism as biocatalyst. Also provided is an aqueous amide compound obtained by the methods of the invention as well as a composition comprising acrylamide or polyacrylamide as well as a dried microorganism exhibiting a NHase/Amidase activity ratio of at least 400 when being brought into contact with a nitrile compound to convert said nitrile compound into an amide compound.
A method for producing an amide compound from a nitrile compound, comprising contacting a nitrile compound with a Nitrile hydratase (NHase) and Amidase producing microorganism, wherein said microorganism is pre-treated by a drying step before being contacted with said nitrile compound.
A method for producing an amide compound from a nitrile compound, comprising the steps of:
A method for producing a microorganism with an increased NHase/Amidase activity ratio, comprising drying a NHase and Amidase producing microorganism.
A method for producing a microorganism with a decreased Amidase activity, comprising drying a NHase and Amidase producing microorganism.
A composition comprising acrylamide or polyacrylamide and a NHase and Amidase producing microorganism, said microorganism exhibiting a NHase/Amidase activity ratio of at least 400 and/or a ratio of the NHase activity to Amidase activity which is increased by a factor of at least 1.7, when compared to a reference microorganism.
28-30. (canceled)
Dependencies are inferred from the claim text.