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AssigneeMonsanto Technology LLC
InventorsJohn D. Bradley, Brandi J. Chiapelli, Bingli Gao, James P. McCarter, Michelle L. Gasper, Deryck Jeremy Williams
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The present invention discloses gene targets, constructs and methods for the genetic control of plant disease caused by nematodes of the genus Meloidogyne (root knot nematodes). The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences in the cells of plant-parasitic nematodes. The disclosed gene targets show significant conservation at the nucleotide level between orthologs from different Meloidogyne species, facilitating genus-wide targeting by RNA interference.
A polynucleotide encoding a double stranded ribonucleotide molecule, the ribonucleotide molecule comprising:
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A method for controlling a plant-parasitic nematode population comprising providing an agent comprising a first polynucleotide sequence comprising from about 19 to about 25 contiguous nucleotides of a sequence is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:32, SEQ ID NO:40, SEQ ID NO:42, SEQ ID NO:44, SEQ ID NO:46, and SEQ ID NO:47, and the complements thereof, wherein the first polynucleotide is hybridized to a second polynucleotide sequence that is complementary to the first polynucleotide sequence.
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Dependencies are inferred from the claim text.