Methods, systems, and compositions for cell-derived/vesicle-based microrna delivery
AssigneeHenry Ford Health System
InventorsMark E. Katakowski, Benjamin A.L. Buller, Michael Chopp
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Abstract
Some embodiments comprise methods, systems, and compositions to produce and/or administer modified exosomes or other vesicles containing one or more selected microRNAs, including but not limited to, miR-146b. Some embodiments also comprise the therapeutic administration and use of such modified exosomes and/or producer cells to treat mammalian brain or neurologic injuries and diseases, including in human beings.
Claims21
A method of treating a subject suffering from a neurological disease or injury with exosomes containing miR-146b microRNA, comprising the steps of:
- harvesting exosomes containing miR-146b microRNA from a cell population capable of producing exosomes containing miR-146b microRNA or media containing the cell population,
- confirming the presence of the miR-146b microRNA in the harvested exosomes;
- and
- administering to the subject in need thereof, the harvested exosomes in a pharmaceutically effective amount to treat the subject with the neurological disease or injury.
A method of treating a subject suffering from a neurological disease or injury with cells capable of producing miR-146b microRNA, the method comprising:
- providing a cell population capable of producing exosomes containing miR-146b;
- confirming the presence of the miR-146b microRNA in the cell population, and
- administering to the subject in need thereof the cell population capable of producing exosomes containing miR-146b microRNA in a pharmaceutically effective amount to treat the subject with respect to the neurological disease or injury.
A method of treating a subject suffering from dementia, neuropathy, stroke or multiple sclerosis with exosomes containing miR-146b microRNA, the method comprising:
- harvesting exosomes containing miR-146b microRNA from a cell population capable of producing the exosomes or media containing the cell population;
- confirming the presence of the miR-146b microRNA in the harvested exosomes;
- and
- administering to the subject in need thereof, the harvested exosomes containing miR-146b microRNA in a pharmaceutically effective amount to treat the subject with the dementia, neuropathy, stroke or multiple sclerosis.
A method of treating a subject suffering from stroke with exosomes containing miR-146b comprising the steps of:
- harvesting exosomes containing miR-146b microRNA from a cell population capable of producing exosomes containing miR-146b microRNA or media containing the cell population,
- confirming the presence of the miR-146b microRNA in the harvested exosomes;
- and
- administering to the subject in need thereof, the harvested exosomes in a pharmaceutically effective amount to treat the subject with the stroke.
Dependencies are inferred from the claim text.