Antiviral nuclease methods
AssigneeAgenovir Corporation
InventorsDerek D. Sloan, Xin Cindy Xiong, Stephen R. Quake
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Abstract
Methods and compositions treat a viral infection use a nuclease and an inhibitor that prevents DNA repair, such as a CRISPR-associated nuclease and a small molecule that inhibits an enzyme of a repair pathway. Under guidance of a targeting sequence, the nuclease cuts viral nucleic acid without cutting the patient's genome. The cut ends of the viral nucleic acid are not repaired because the inhibitor prevents a repair mechanism.
Claims28
A system for targeted treatment of a viral infection, the system comprising:
- a nuclease capable of cutting viral nucleic acid into fragments;
- a targeting sequence that targets the nuclease to the viral nucleic acid;
- and
- a DNA repair inhibitor.
A composition for targeted treatment of nucleic acid, the composition comprising:
- a vector encoding a nuclease that cuts target nucleic acid into fragments and a targeting sequence that targets the nuclease to the target nucleic acid;
- and
- a DNA repair inhibitor.
A method for targeted cutting of viral nucleic acid, the method comprising:
- introducing into a host cell:
- a nuclease, a targeting sequence that targets the nuclease to the viral nucleic acid, and a DNA repair inhibitor;
- targeted cutting, by the nuclease, of the viral nucleic acid into fragments;
- and
- preventing, via the DNA repair inhibitor, ligation of ends of the fragments.
Dependencies are inferred from the claim text.