Artificial nucleic acid molecules for improved protein or peptide expression
AssigneeCureVac AG
InventorAndreas THESS
Life of this patent
Estimating the term…
Abstract
The invention relates to an artificial nucleic acid molecule comprising at least one 5′UTR element which is derived from a TOP gene, at least one open reading frame, and preferably at least one histone stem-loop. Optionally the artificial nucleic acid molecule may further comprise, e.g. a poly(A)sequence, a poyladenylation signal, and/or a 3′UTR. The invention further relates to the use of such an artificial nucleic acid molecule in gene therapy and/or genetic vaccination.
Claims101
An artificial nucleic acid molecule comprising:
- a. at least one 5′-untranslated region element (5′UTR element) which comprises or consists of a nucleic acid sequence which is derived from the 5′UTR of a TOP gene or which is derived from a variant of the 5′UTR of a TOP gene;
- and
- b. at least one open reading frame (ORF).
A vector comprising:
- a. at least one 5′-untranslated region element (5′UTR element) which comprises or consists of a nucleic acid sequence which is derived from the 5′UTR of a TOP gene or which is derived from a variant of the 5′UTR of a TOP gene;
- and
- b. at least one open reading frame (ORF) and/or at least one cloning site.
A method for increasing protein production from an artificial nucleic acid molecule, comprising the step of providing the artificial nucleic acid molecule with
- i. at least one 5′-untranslated region element (5′UTR element) which comprises or consists of a nucleic acid sequence which is derived from the 5′UTR of a TOP gene or which is derived from a variant of the 5′UTR of a TOP gene;
- ii. preferably at least one histone stem-loop;
- and
- iii. optionally, a poly(A) sequence and/or a polyadenylation signal.
Use of a 5′UTR element which comprises or consists of a nucleic acid sequence which is derived from the 5′UTR of a TOP gene or which is derived from a variant of the 5′UTR of a TOP gene and preferably at least one histone stem-loop for increasing protein production from a nucleic acid molecule.
Dependencies are inferred from the claim text.