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US 2017/0247679 A1Published application
Blends containing proteases
AssigneeRoche Diagnostics Operations, Inc.
InventorsWerner Hoelke, Michaela Fischer, Johann-Peter Thalhofer, Markus Weber
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Abstract
Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.
Claims10
A method for producing a solid composition comprising the steps of:
- preparing a homogeneous solution of an acetate-free preparation of a neutral protease (NP) in an aqueous acetate-free, low-salt solution, wherein the low-salt solution has an aggregate concentration of salt(s) in the range of about 1 mM to about 250 mM;
- adding a neutral salt to the homogeneous solution and dissolving the neutral salt, thereby making a stabilized solution, wherein the stabilized solution additionally comprises a buffer salt buffering in a range of about pH 6 to about pH 8.5, and wherein the stabilized solution further comprises calcium chloride;
- mixing the stabilized solution with an acetate-free preparation of one or more proteolytic enzymes with collagenase activity (C) and making a homogeneous solution;
- and
- freeze-drying the homogeneous solution, thereby obtaining the solid composition.
2 more elements
Dependencies are inferred from the claim text.