Alpha amylases in feed
AssigneeDupont Nutrition Biosciences Aps
InventorMAI FAURSCHOU ISAKSEN
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Abstract
The present invention relates to a method for identifying a pepsin resistant alpha amylase enzyme for use in a feed supplement comprising: i) providing an alpha amylase enzyme; ii) admixing said alpha amylase with com based feed and buffer solution comprising a pepsin concentration of 9000 U/ml at pH 3, 40° C., 500 rpm for al least 120 minutes and analysing alpha amylase activity on said alpha amylase compared to a control sample; wherein said control sample differs in that no pepsin is present during incubation; and iii) selecting an alpha amylase enzyme which substantially maintains alpha amylase activity under the assay conditions; feed supplements and feed stuffs comprising a pepsin resistant alpha amylase and the use of pepsin resistant alpha amylases in feed.
Claims17
A method for identifying a pepsin resistant alpha amylase enzyme for use in a feed supplement comprising:
- i) providing an alpha amylase enzyme;
- ii) admixing said alpha amylase with corn based feed and pepsin, incubating at pH 3 and analysing the alpha amylase activity of said alpha amylase compared to a control sample;
- wherein said control sample differs in that no pepsin is present during the incubation at pH 3;
- and
- iii) selecting an alpha amylase enzyme which substantially maintains alpha amylase activity under the assay conditions.
A method for preparing a feed supplement for a monogastric animal comprising admixing a pepsin resistant alpha amylase with at least one physiologically acceptable carrier selected from the group consisting of maltodextrin, limestone (calcium carbonate), cyclodextrin, wheat or a wheat component, sucrose, starch, Na2SO4, Talc, PVA, sorbitol, benzoate, sorbiate, glycerol, sucrose, propylene glycol, 1,3-propane diol, glucose, parabens, sodium chloride, citrate, acetate, phosphate, calcium, metabisulfite, formate and mixtures thereof.
13-32. (canceled)
A method for preparing a feedstuff for a monogastric animal comprising mixing feed supplement according to claim 13 with one or more feed materials.
35-36. (canceled)
37-70. (canceled)
Dependencies are inferred from the claim text.