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US 2017/0247670 A1Published application
Thermostable Variants of T7 RNA Polymerase
AssigneeNew England Biolabs, Inc.
InventorsJennifer Ong, Vladimir Potapov, Kuo-Chan Hung, Haruichi Asahara, Shaorong Chong, George Tzertzinis
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Abstract
A bacteriophage RNA polymerase variant is provided. In some embodiments, the variant may have increased thermostability relative to the corresponding wild type bacteriophage RNA polymerase and/or wild type T7 RNA polymerase. Compositions, kits and methods that employ the variant are also provided.
Claims25
An isolated bacteriophage RNA polymerase variant, wherein the variant:
- (i) comprises an amino acid sequence is at least 80% sequence identity to SEQ ID NO:1;
- and
- (ii) comprises an amino acid substitution at one or more positions corresponding to positions selected from 109, 205, 388, 534, 567 and 618 of SEQ ID NO:1.
Dependencies are inferred from the claim text.