HELICOBACTER PYLORI a-1,3 FUCOSYLTRANSFERASE GENE AND PROTEIN WITH IMPROVED SOLUBLE PROTEIN EXPRESSION AND ACTIVITY, AND THEREOF APPLICATION FOR SYNTHESIS OF a-1,3 FUCOSYLOLIGOSACCHARIDE

An α-1,3 fucosyltransferase mutant represented by any one sequence selected from (a) to (p):

• (a) an amino acid sequence of SEQ ID NO:
• 1;
• (b) an amino acid sequence of SEQ ID NO:
• 2;
• (c) an amino acid sequence of SEQ ID NO:
• 3;
• (d) an amino acid sequence having a homology of 95% or more to the amino acid sequence of any one of SEQ ID NOS:
• 1 to 3 and having fucosyltransferase activity;
• (e) a sequence wherein the amino acid at position 128 of any one of SEQ ID NOS:
• 1 to 3 is substituted with an amino acid other than alanine and aspartic acid;
• (f) a sequence wherein the amino acid at position 129 of any one of SEQ ID NOS:
• 1 to 3 is substituted with an acidic hydrophilic amino acid;
• (g) a sequence wherein the amino acid at position 132 of any one of SEQ ID NOS:
• 1 to 3 is substituted with an amino acid other than tyrosine and histidine;
• (h) a sequence wherein the amino acid at position 46 of any one of SEQ ID NOS:
• 1 to 3 is substituted with a hydrophobic amino acid;
• (i) a sequence wherein the amino acid at position 128 of any one of SEQ ID NOS:
• 1 to 3 is substituted with an amino acid other than alanine and aspartic acid and the amino acid at position 129 is substituted with an acidic hydrophilic amino acid;
• (j) a sequence wherein the amino acid at position 128 of any one of SEQ ID NOS:
• 1 to 3 is substituted with an amino acid other than alanine and aspartic acid and the amino acid at position 132 is substituted with an amino acid other than tyrosine and histidine;
• (k) a sequence wherein the amino acid at position 128 of any one of SEQ ID NOS:
• 1 to 3 is substituted with an amino acid other than alanine and aspartic acid and the amino acid at position 46 is substituted with a hydrophobic amino acid;
• (l) a sequence wherein the amino acid at position 128 of any one of SEQ ID NOS:
• 1 to 3 is substituted with an amino acid other than alanine and aspartic acid, the amino acid at position 129 is substituted with an acidic hydrophilic amino acid, and the amino acid at position 132 is substituted with an amino acid other than tyrosine and histidine;
• (m) a sequence wherein the amino acid at position 128 is substituted with an amino acid other than alanine and aspartic acid, the amino acid at position 129 is substituted with an acidic hydrophilic amino acid, and the amino acid at position 46 is substituted with a hydrophobic amino acid;
• (n) a sequence wherein the amino acid at position 128 of any one of SEQ ID NOS:
• 1 to 3 is substituted with an amino acid other than alanine and aspartic acid, the amino acid at position 132 is substituted with an amino acid other than tyrosine and histidine, and the amino acid at position 46 is substituted with a hydrophobic amino acid;
• (o) a sequence wherein the amino acid at position 128 of any one of SEQ ID NOS:
• 1 to 3 is substituted with an amino acid other than alanine and aspartic acid, the amino acid at position 129 is substituted with an acidic hydrophilic amino acid, the amino acid at position 132 is substituted with an amino acid other than tyrosine and histidine, and the amino acid at position 46 is substituted with a hydrophobic amino acid;
• and
• (p) a sequence wherein the amino acid at one or more positions selected from the group consisting of positions 128, 129, 132 and 46 of any one of SEQ ID NOS:
• 1 to 3 is substituted with another amino acid.