Myocardial cell sheet
AssigneesTerumo Kabushiki Kaisha, Osaka University
InventorsHiroko ISEOKA, Yoshiki SAWA, Shigeru MIYAGAWA, Satsuki FUKUSHIMA
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Abstract
A sheet-shaped cell culture is disclosed that includes myocardial cells derived from pluripotent stem cells and has excellent functional properties. A sheet-shaped cell culture obtained by culturing a cell population that includes myocardial cells derived from pluripotent stem cells, wherein the proportion of the number of the myocardial cells derived from pluripotent stem cells to the total number of cells in the cell population is 50% to 70%; a method of producing the sheet-shaped cell culture; a composition including the sheet-shaped cell culture; and a method of treating a disease using the sheet-shaped cell culture or the composition.
Claims20
A sheet-shaped cell culture obtained by culturing a cell population, the sheet-shaped cell culture comprising:
- myocardial cells derived from pluripotent stem cells, wherein a proportion of a number of the myocardial cells derived from pluripotent stem cells to a total number of cells in the cell population is 50% to 70%.
A method of enhancing a cytokine producing ability of a sheet-shaped cell culture including myocardial cells derived from pluripotent stem cells, the method comprising:
- (1) a step of subjecting pluripotent stem cells to a myocardial cell derivation treatment to obtain a first cell population including myocardial cells;
- (2) a step of obtaining a second cell population including the myocardial cells derived from the first cell population obtained in the step (1);
- and
- (3) a step of culturing the second cell population obtained in the step (2) to form a sheet-shaped cell culture,
- wherein a proportion of a number of the myocardial cells to a total number of cells in the second cell population is 50% to 70%.
Dependencies are inferred from the claim text.