Novel method for culture of microalgae

Method for producing a biomass of microalgae comprising the steps of a) culturing the microalgae in a culture medium and b) harvesting the biomass from the culture medium, wherein step a) of culturing the microalgae comprises at least a first step

• a1) wherein said microalgae are cultivated in a defined medium, comprising
• macroelements comprising a mixture of sodium chloride (NaCl), magnesium chloride, sodium bicarbonate (NaHCO3), potassium hydrogen phosphate (K2HPO4), calcium chloride, iron (III) chloride (FeCl3 6H2O), boric acid (H3BO3) and a nitrogen source;
• microelements comprising a mixture of disodium ethylenediaminetetraacetic acid (Na2EDTA), manganese (II) chloride tetrahydrate (MnCl2 4H2O), zinc chloride (ZnCl2), cobalt chloride hexahydrate (CoCl2 6H2O), copper sulfate pentahydrate (CuSO4 5H2O), and nickel sulfate hexahydrate (NiSO4 6H2O);
• vitamins comprising a mixture of biotin, thiamin, cobalamin or vitamin B12 and pantothenate or vitamin B5;
• and a carbon source;
• for a sufficient time to reach a strain concentration between 5×106 and 5×108 cells/mL, advantageously between 107 and 108 cells/mL,
• and at least a second step
• a2) wherein one or more carbon source enrichment solution(s) are added, simultaneously or successively, to the culture medium and wherein the microalgae is a microalgae strain having a gene encoding the small subunit 18s ribosomal RNA having a genetic similarity of at least 96% with the same gene of the strain of the species Crypthecodinium cohnii deposited in the CCAP (Culture Collection of Algae and Protozoa) under number CCAP 1104/3.