Back to results
US 2017/0247652 A1Published application
Device and Method for High Throughput Bacterial Isolation
AssigneeNortheastern University
InventorsEdgar D. GOLUCH, Nil TANDOGAN
Life of this patent
Estimating the term…
Abstract
Devices and methods for isolating and characterizing microbial cells from an environment are provided. The devices integrate sub-micron constrictions in a nanofluidic device with a standard microtiter plate format to facilitate the high throughput isolation, culturing, analysis, and screening of bacteria and other microbial cells in natural and man-made environments, particularly environments containing microbes adhered on particulate matter.
Claims65
A device for isolating and culturing single cells of a population of microbial cells from an environment, the device comprising:
- a nanochannel comprising a first end disposed at a surface of the device exposed to an environment, the environment comprising a mixture of microbial cells;
- and
- a food chamber fluidically coupled to a second end of the nanochannel;
- wherein the nanochannel has a cross-sectional diameter that allows entry of only a single microbial cell from the mixture of microbial cells and prevents the microbial cell from entering the food chamber, but allows only progeny of the single microbial cell to enter the food chamber.
1 more element
Dependencies are inferred from the claim text.