Mammalian cell culture performance through surfactant supplementation of feed media
AssigneeAbbVie Inc.
InventorsSean McDermott, Patrick Hossler
Life of this patent
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Abstract
The present invention provides methods for increasing cell culture performance through the use of chemically defined feed media (CDFM). In particular, the present invention provides methods for the use of surfactants as supplements to CDFM to allow for higher concentrations of media components and thereby result in increased cell culture performance.
Claims40
A method of increasing cell culture performance, the method comprising:
- (a) culturing a cell line that expresses a protein of interest in a culture media;
- and
- (b) supplementing said culture media with a chemically defined feed media (CDFM) comprising a surfactant,
- wherein the surfactant is present in an amount sufficient to achieve increased cell culture performance, thereby increasing cell culture performance.
A chemically defined feed media (CDFM) comprising a surfactant in an amount sufficient to reduce amino acid aggregation.
An antibody, or antigen-binding portion thereof, wherein said antibody, or antigen-binding portion thereof, is produced from cells grown in a culture media supplemented with a chemically defined feed media (CDFM) comprising a surfactant, and wherein the antibody, or antigen-binding portion thereof, comprises a decrease in high molecular weight (HMW) species by about 2.6% relative to said antibody, or antigen-binding portion thereof, when produced from cells grown in the culture media not supplemented with CDFM comprising the surfactant.
Dependencies are inferred from the claim text.