Anti-ang2 antibodies and methods of use

An antibody that specifically binds to human ANG2, wherein the antibody

• i) comprises a heavy chain variable domain that has a sequence identity to SEQ ID NO:
• 19 of more than 70% and a light chain variable domain that has a sequence identity to SEQ ID NO:
• 6 of more than 70%,
• ii) has in the heavy chain variable domain at position 28 the amino acid residue asparagine (N), at position 30 the amino acid residue alanine (A), at position 100b the amino acid residue proline (P) and at position 100j the amino acid residue alanine (A) and in the light chain variable domain at position 51 the amino acid residue threonine (T) (numbering according to Kabat), and
• iii) the antibody has a lower EC50 value for the inhibition of the binding of ANG2 to its receptor Tie2 in a cell based assay using HEK293 cells stably expressing human Tie2 determined using a Tie2 phosphorylation ELISA compared to an antibody comprising a heavy chain variable domain that has the sequence of SEQ ID NO:
• 19 and a light chain variable domain that has the sequence of SEQ ID NO:
• 6 or SEQ ID NO:
• 33.