IMPROVED PROCESS FOR THE PREPARATION OF IMMUNOGLOBULIN G (IgG)
AssigneeBio Products Laboratory Limited
InventorsJohn MORE, Tara DOLAN
Life of this patent
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Abstract
The present invention is directed to processes for extracting IgG from an unused waste precipitate produced during normal plasma fractionation processes via a separate fractionation process, thereby increasing the overall yield of IgG from blood plasma.
Claims24
A method for extracting IgG from a waste precipitate fraction, the method comprising contacting the waste precipitate fraction with a suitable solvent to extract IgG from the precipitate, wherein the waste precipitate fraction is produced during plasma fractionation and separated from the main IgG manufacturing process stream.
A method for improving the yield of IgG during plasma fractionation to produce IgG, the method comprising extracting IgG from a waste precipitate fraction, wherein the waste precipitate fraction is produced during plasma fractionation and separated from the main IgG manufacturing process stream using a suitable solvent.
A method for the separation of IgG from plasma or a plasma fraction, the method comprising
- a) fractionating the plasma or plasma fraction to produce a liquid fraction containing a majority of the IgG present in the plasma or plasma fraction, and a waste precipitate fraction containing additional IgG;
- and
- b) extracting at least some of the additional IgG from the waste precipitate fraction using a suitable solvent.
A method for the preparation of IgG comprising:
- a) recovering precipitate and supernatant from a modified Kistler and Nitschmann B+I fractionation process;
- b) homogenising the precipitate obtained in step a) and extracting IgG therefrom by mixing with 17 vol. % aqueous ethanol in acetate/phosphate buffer at 0° C. for 1 to 3 hours;
- and then
- c) separating the buffer containing extracted IgG from any remaining precipitate.
Dependencies are inferred from the claim text.