Display of binding agents
AssigneeF-Star Biotechnologische Forschungs-Und Entwicklungsges.M.B.H.
InventorsGottfried HIMMLER, Geert MUDDE, Gerda REDL, Florian RUKER, Gordana WOZNIAK-KNOPP
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Abstract
The present application relates to a method of preparing a genetic package displaying oligomers of modular antibody domains binding to a target and to a scaffold ligand as well as to vectors and libraries of genetic packages produced thereby. The invention further relates to methods of selecting suitable linker sequences for use in such oligomer display.
Claims8
A method of producing a functional binding agent, wherein the functional binding agent binds to a target antigen, wherein said target antigen is a receptor of the erbB class, wherein the functional binding agent is an antibody Fc fragment comprising a CH2 domain and a CH3 domain, comprising the steps of:
- a. providing a library of said antibody Fc fragments,
- b. contacting said library with said target antigen in the presence of a scaffold ligand, wherein said target antigen and said scaffold ligand are different molecules, wherein said scaffold ligand is selected from the group consisting of CD64, CD16, CD32, FcRn and Protein A,
- c. selecting a library member binding to said target antigen in the presence of said scaffold ligand to obtain a functional binding agent, and
- d. producing a preparation of the functional binding agent.
Dependencies are inferred from the claim text.