Methods of Generating Bioactive Peptide-bearing Antibodies and Compositions Comprising the Same
AssigneeOmeros Corporation
InventorsW. Jason Cummings, Patrick Gray, Larry Tjoelker, Munehisa Yabuki
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Abstract
In one aspect, the invention provides a method of making a bioactive peptide-bearing antibody, or fragment thereof, comprising (a) engrafting the amino acid sequence of at least one bioactive peptide of interest into (i) at least one of CDR-H1, CDR-H2 or CDR-H3 of a heavy chain variable region comprising one or more chicken framework regions and/or (ii) at least one of CDR-L1, CDR-L2 or CDR-L3 of the light chain variable region comprising one or more chicken framework regions, and (b) determining whether the antibody has substantially the same biological activity as the bioactive peptide.
Claims40
-26. (canceled)
An isolated antibody, or antigen-binding fragment thereof, comprising one or more bioactive peptide amino acid sequence(s), wherein at least one bioactive peptide amino acid sequence is engrafted into at least one of:
- (i) a light chain variable region comprising one or more chicken framework regions and/or (ii) a heavy chain variable region comprising one or more chicken framework regions.
-56. (canceled)
A method of making a bioactive peptide-bearing antibody, comprising:
- (a) engrafting the amino acid sequence of at least one bioactive peptide of interest into:
- (i) at least one of CDR411, CDR-H2 or CDR-H3 of a heavy chain variable region comprising one or more chicken framework regions and/or
- (ii) at least one of CDR-L1, CDR-L2 or CDR-L3 of the light chain variable region comprising one or more chicken framework regions, and
- (b) determining whether the antibody has substantially the same biological activity as the bioactive peptide.
-71. (canceled)
-85. (canceled)
Dependencies are inferred from the claim text.