Affinity chromatography matrix
AssigneeGE Healthcare BioProcess R&D AB
InventorsHans J. Johansson, Ronnie Palmgren
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Abstract
The invention discloses a polypeptide capable of binding immunoglobulins or immunoglobulin-containing proteins, which polypeptide comprises six or more domains of protein Z or the C domain of protein A or a functional variant thereof. It also discloses separation matrices comprising the polypeptide and methods of using the separation matrices for separation of immunoglobulins or immunoglobulin-containing proteins.
Claims20
A separation matrix comprising a plurality of chromatography ligands coupled to a solid support,
- wherein the ligand comprises one or more mutated Domain C of Staphylococcus protein A (SpA) and having a sequence that is at least 80% identical to the amino acid sequence shown in SEQ ID NO:1 or at least 77% identical to the amino acid sequence shown in SEQ ID NO:2.
A separation matrix comprising a plurality of chromatography ligands coupled to a solid support,
- wherein the ligand comprises one or more mutated Domain C of Staphylococcus protein A (SpA) and having a sequence selected from SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 1 with mutation G29A, and SEQ ID NO:
- 2 with mutation G25A.
Dependencies are inferred from the claim text.