Method and apparatus for the filtration of biological solutions
AssigneeSpf Innovations, LLC
InventorsGaston de los Reyes, Leon Mir
Life of this patent
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Abstract
A system, method and device are disclosed for bio-processing a feed stream and providing a constant output by operating a continuous single-pass tangential-flow process. The single-pass process provides high conversion concentration while operating at relatively low feed flow rates, and the process can also be used to provide constant output diafiltration.
Claims12
A filtration system comprising:
- a plurality of stages, each stage having a plurality of channels providing at least one serial flow path, each stage being in fluid communication with each adjacent stage preceding it and being in fluid communication with each adjacent stage that follows it;
- each of the plurality of channels comprising a filtration membrane and having a length, a membrane area, a void volume, a specific membrane area expressed as a ratio of the membrane area to the void volume, and a dimensionless length expressed as a product of the channel length and the specific membrane area;
- wherein a dimensionless length of a stage is the sum of the dimensionless lengths of each channel in the longest serial flow path in the stage and the dimensionless length of the system is the sum of the dimensionless lengths of the plurality of stages;
- wherein the specific membrane area of at least one channel is greater than about 40 cm−1, the dimensionless length of the system is greater than about 2,000 and the dimensionless length of at least one of the plurality of stages is less than about 6,000;
- and
- wherein at least one of the plurality of stages comprises a cassette.
A staging plate for serializing the flow through a plurality of cassettes arranged in stages, each cassette having a feed manifold and a retentate manifold, the staging plate comprising:
- a plate;
- a pass through port disposed in the plate to align with corresponding ones of the feed manifold of the plurality of cassettes and corresponding ones of the retentate manifold of the plurality of cassettes;
- a blocking point to block the feed stream from entering an adjacent stage;
- and
- wherein the staging plate serializes the flow from at least one cassette of the plurality of cassettes.
A method for converting a plurality of cassettes from parallel flow to serial flow comprising:
- stacking a plurality of cassettes;
- placing a device for serializing the flow, adjacent to at least one cassette in the stacked plurality of cassettes.
Dependencies are inferred from the claim text.