Supercritical fluid chromatography system

A system comprising:

• a) a tank comprising a gas stored at saturated conditions and a liquid withdrawal means;
• b) a chiller in fluid communication with the tank, wherein the chiller subcools the gas to a temperature of −10° C. or lower;
• c) a pump downstream of and in fluid communication with the tank and the chiller, and a chamber configured for liquefied gas or supercritical gas extraction, wherein the pump comprises the gas at a temperature of −10° C. or lower;
• wherein the mass flow rate of the subcooled liquid phase gas through the pump is repeatable and proportionate to pump speed.

A cyclonic separator comprising:

• a) a cyclone body comprising an inner surface, an outer circumference, a top outlet, a tangential inlet and a bottom outlet, wherein the inner surface comprises a top portion, a middle portion and a bottom portion, wherein:
• i) the top portion of the inner surface comprises screw threads;
• ii) the middle portion of the inner surface is cylindrical;
• iii) the bottom portion of the inner surface comprises a funnel, wherein the funnel has an angle in the range of about 30° to about 60°;
• and wherein the ratio of the diameter of the outer circumference to the inner diameter of the mid-height of the funnel is in the range of about 3 to about 4;
• and
• b) a cap comprising a sintered filter and screw threads, wherein the screw threads of the cap interlock with the screw threads on the inner surface of the top portion of the cyclone body, wherein the cyclonic separator can withstand pressures of at least about 1000 psi, and wherein the body is in fluid communication with the cap.