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US 2017/0246350 A1Published application
"Bioengineered Human Corneal Stromal Tissue"
AssigneeUniversity of Pittsburgh—Of the Commonwealth System of Higher Education
InventorsYiqin Du, James L. Funderburgh, William R. Wagner, Jian Wu
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Abstract
Provided herein is a method of making an aligned ECM scaffold useful in refractive correction of the eye and repair of the cornea. Methods of use of the scaffold as well as a scaffold construct are provided.
Claims20
A biological scaffold comprising:
- a plurality of layers of aligned fibers of a biocompatible, biodegradable polymeric composition, each layer having keratocyes or keratocytes precursors seeded thereon, the polymeric composition comprising:
- a polyurethane, a polyester, a polyether, a polyacrylamide, and/or a polycarbonate;
- a polyurethane, a polyester, a polyether, a polyacrylamide, and/or a polycarbonate-containing block copolymer, or
- a copolymer formed from one or more acrylic monomers, acrylamide monomers, succinimide monomers, glycolide monomers, caprolactone monomers, dioxanone monomers, lactide monomers, and/or carbonate monomers,
- wherein the aligned fibers of a first layer of the plurality of layers are arranged orthogonally with respect to the aligned fibers of a second layer of the plurality of layers that is adjacent to the first layer.
2 more elements
A method of changing the refractive power of a cornea in an eye of a patient comprising:
- providing a template scaffold comprising:
- a plurality of layers of aligned fibers of a biocompatible, biodegradable polymeric composition, each layer having keratocyes or keratocytes precursors seeded thereon, the polymeric composition comprising:
- a polyurethane, a polyester, a polyether, a polyacrylamide, and/or a polycarbonate;
- a polyurethane, a polyester, a polyether, a polyacrylamide, and/or a polycarbonate-containing block copolymer, or
- a copolymer formed from one or more acrylic monomers, acrylamide monomers, succinimide monomers, glycolide monomers, caprolactone monomers, dioxanone monomers, lactide monomers, and/or carbonate monomers,
- wherein the aligned fibers of a first layer of the plurality of layers are arranged orthogonally with respect to the aligned fibers of a second layer of the plurality of layers that is adjacent to the first layer;
- allowing the aligned fibers to degrade, leaving a product ECM scaffold comprising the keratocytes or keratocytes precursors and any extracellular matrix (ECM) generated thereby;
- and
- onlaying or inlaying the product ECM scaffold in the cornea of a patient in need of an alteration of refractive corneal power.
6 more elements
A method of adding stromal tissue to a cornea of a patient, comprising:
- providing a template scaffold comprising:
- a plurality of layers of aligned fibers of a biocompatible, biodegradable polymeric composition, each layer having keratocyes or keratocytes precursors seeded thereon, the polymeric composition comprising:
- a polyurethane, a polyester, a polyether, a polyacrylamide, and/or a polycarbonate;
- a polyurethane, a polyester, a polyether, a polyacrylamide, and/or a polycarbonate-containing block copolymer, or
- a copolymer formed from one or more acrylic monomers, acrylamide monomers, succinimide monomers, glycolide monomers, caprolactone monomers, dioxanone monomers, lactide monomers, and/or carbonate monomers,
- wherein the aligned fibers of a first layer of the plurality of layers are arranged orthogonally with respect to the aligned fibers of a second layer of the plurality of layers that is adjacent to the first layer;
- allowing the aligned fibers to degrade, leaving a product ECM scaffold comprising the keratocytes or keratocytes precursors and any extracellular matrix (ECM) generated thereby;
- and
- onlaying or inlaying the product ECM scaffold in the cornea of the patient in need of corneal repair.
6 more elements
Dependencies are inferred from the claim text.