US 2017/0246313 A1 · AN IMPROVED PROCESS OF CONJUGATION AND NOVEL SYNTHETIC OLIGOSACCHARIDE- PROTEIN CONJUGATES OBTAINED THEREOF

Abstract

The present invention relates to an improved process of conjugation to obtain synthetic oligosaccharide-protein (OS-PR) conjugates. The process of synthetic OS-PR conjugation is a rapid process providing oligosaccharide-protein conjugates which are highly immunogenic and elicit specific and homogenous immune responses. The synthetic oligosaccharide comprising of four to eight repeating units of respective monomers and at least one in-built terminal amino linker, said synthetic polysaccharide mimics natural polysaccharide obtained from gram negative bacteria such as Neisseria meningitidis serogroups A, C, Y, W, X and Haemophilus influenzae and carrier protein is obtained from gram positive bacteria such as Clostridium tetani (tetanus toxoid) or Corynebacterium diphtheriae (CRM197) or their recombinant versions. The conjugation chemistry of the said oligosaccharide-protein conjugate of the present invention is thio-ether linkage. The present invention takes complete process time in the range of 14-22 hours. The said oligosaccharide-protein conjugates are useful in production of monovalent vaccine or multivalent combination vaccines and as diagnostic tool.

Claims15

Dependent claims

An improved process of conjugation to obtain novel synthetic oligosaccharide-protein conjugates comprising the steps of:

(a) reacting a synthetic oligosaccharide having at least one terminal amine linker with a reagent having at least one reactive thiol functional group to obtain an activated oligosaccharide;
(b) reacting a carrier protein with at least one aliphatic hetero bifunctional crosslinker to obtain an activated carrier protein having reactive maleimide functional group;
(c) carrying out conjugation reaction of said activated synthetic oligosaccharide of step (a) with said activated carrier protein of step (b) to obtain a synthetic oligosaccharide-protein conjugate (OS-PR) having thioether linkage;
wherein
said process of conjugation is completed in a range of 14 hours to 22 hours,
said process results into higher yields of oligosaccharide-protein conjugate vis a vis yields from conventional conjugates,
said oligosaccharide-protein conjugate displays significantly high antibody titer including functional antibodies.

4 more elements

Dependencies are inferred from the claim text.

An improved process of conjugation and novel synthetic oligosaccharide- protein conjugates obtained thereof

Life of this patent

An improved process of conjugation and novel synthetic oligosaccharide- protein conjugates obtained thereof

Life of this patent

Abstract

Claims15