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US 2017/0246277 A1Published application
Reagents and methods for identifying, enriching, and/or expanding antigen-specific t cells
AssigneeThe Johns Hopkins University
InventorsJonathan Schneck, Karlo Perica, Joan Glick Bieler, Mathias Oelke
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Abstract
Antigen-specific T cells, including nave T cells, and including rare precursor cells are enriched and expanded in culture. Enrichment and expansion provides a platform for more effective immunotherapy by adoptive transfer, as well as platforms for personalizing immunotherapy by determining T cell reactivity with a library of candidate peptide antigens.
Claims83
A method for preparing an antigen-specific T-cell population, comprising:
- providing a sample comprising T-cells from a patient or a suitable donor;
- contacting said sample with a population of first particles which are paramagnetic and comprise on their surfaces an MHC-peptide antigen-presenting complex, wherein the particles are from about 10 to about 500 nm in diameter, and
- placing a magnetic field in proximity to the paramagnetic particles, and
- recovering antigen-specific T-cells associated with the magnetic particles.
1 more element
A method for identifying a T cell antigen or component of a T cell repertoire, comprising:
- providing a sample comprising T cells from a subject,
- screening the isolated T cells against a library of paramagnetic particles each comprising on their surface an MHC-peptide antigen-presenting complex, wherein the particles are from about 10 to about 500 nm in diameter, wherein each particle presents a candidate peptide antigen, and
- identifying one or more peptide antigens that cause activation of the subject's T cells.
Dependencies are inferred from the claim text.