Recombinant listeria strain expressing heterologous antigen fusion proteins and methods of use thereof
OwnerAnu Wallecha, Robert Petit +2inventors
InventorsAnu Wallecha, Robert Petit, Joshua Ellenhorn, Don J. Diamond
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Abstract
Disclosed herein are recombinant nucleic acids encoding tumor antigens fused to immunogenic polypeptides and recombinant Listeria strains comprising the same, methods of preparing same, and methods of inducing an immune response, and treating, inhibiting, or suppressing cancer or tumors comprising administering same.
Claims26
A recombinant nucleic acid molecule comprising an open reading frame encoding a recombinant polypeptide, said recombinant polypeptide comprising a heterologous antigen fused to an N-terminal Listeriolysin O (LLO) polypeptide, wherein said heterologous antigen is survivin, wherein said nucleic acid further comprises a gram-negative origin of replication sequence operably linked to a first promoter sequence, a gram-positive origin of replication sequence, and an open reading frame encoding a metabolic enzyme operably linked to a second promoter sequence.
Dependencies are inferred from the claim text.