Non-human animals having a engineered angptl8 gene
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsViktoria Gusarova, Andrew J. Murphy, Jesper Gromada, Dayong Guo
Life of this patent
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Abstract
Non-human animals, and methods and compositions for making and using the same, are provided, wherein said non-human animals comprise a humanization of an Angiopoietin-like protein 8 (ANGPTL8) gene. Said non-human animals may be described, in some embodiments, as having a genetic modification to an endogenous ANGPTL8 locus so that said non-human animals express a human ANGPTL8 polypeptide.
Claims38
A rodent whose genome comprises an engineered Angptl8 gene that comprises an endogenous Angptl8 sequence and a human ANGPTL8 sequence, and is operably linked to rodent or human Angptl8 regulatory elements.
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A rodent embryonic stem cell whose genome comprises an engineered Angptl8 gene that comprises an endogenous Angptl8 sequence and a human ANGPTL8 sequence, and is operably linked to rodent Angptl8 regulatory elements.
The method of claim 38, wherein said modifying the genome of a rodent comprises
- (a) placing a genomic fragment into an endogenous Angptl8 gene in a rodent embryonic stem cell, said genomic fragment comprising a nucleotide sequence that encodes said human ANGPTL8 polypeptide in whole or in part;
- (b) obtaining the rodent embryonic stem cell generated in (a);
- and,
- (c) creating a rodent using the rodent embryonic stem cell of (b).
-36. (canceled)
A method of making a rodent whose genome comprises an engineered Angptl8 gene that encodes a human ANGPTL8 polypeptide, the method comprising modifying the genome of a rodent so that it comprises an engineered Angptl8 gene that encodes a human ANGPTL8 polypeptide under the control of rodent Angptl8 regulatory sequences, thereby making said rodent.
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Dependencies are inferred from the claim text.